Download Aging in Cell and Tissue Culture: Proceedings of a symposium by Emma Holečková, Vincent J. Cristofalo (auth.), Emma PDF

By Emma Holečková, Vincent J. Cristofalo (auth.), Emma Holečková, Vincent J. Cristofalo (eds.)

The annual assembly of the ecu Tissue tradition ., Society used to be held on the fortress of Zinkovy in Czechoslovakia from may well 7-10,1969. integrated as a part of this assembly used to be a symposium on "Aging in mobilephone and Tissue Culture." This quantity includes the papers provided at that symposium. using telephone and tissue tradition innovations to check the mechanism of getting older isn't new. for instance, it has lengthy been recognized that age-associated adjustments which happen in plasma can inhibit telephone proliferation in vitro; additionally that the time lapse sooner than cellphone migration from ex­ planted tissue fragments raises with expanding age. those are either examples of the expression in vitro of getting older in vivo. extra lately, awareness has been excited by the incidence of senescence in vitro. those investi­ gations have integrated reports of changes in non­ dividing mobile cultures, and to a a little bit larger quantity, of age-related alterations within the proliferative potential of cells in vitro. for instance, cells derived from human fetal lung continue many homes of standard cells together with a solid common diploid karyotype and those cultures were proven to have a constrained life-span in vitro. In addi­ tion, cultures derived from human grownup lung express a similar general features and seem to have a shorter lifestyles­ span than cells derived from fetal lung.

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Extra resources for Aging in Cell and Tissue Culture: Proceedings of a symposium on “Aging in Cell and Tissue Culture” held at the annual meeting of the European Tissue Culture Society at the Castle of Žinkovy in Czechoslovakia, May 7–10, 1969

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There the time between passages was three or four days until passage 21, after which the growth periods were seven days. Thus an increase in the growth periods is correlated with a decrease in (m x s), which could be considered a temporary beneficial effect on the cells, resulting in an extended lifespan. Therefore, this figure indicates that the parameters obtained by size analysis can serve as useful indicators of the physiological age of the cell populations when the cells are cultured under strict constant conditions throughout their Whole lifespan.

Acad. , 49: 517, 1963. P. D. Cooper, "The plaque assay of animal viruses, " in: Methods in Virology, Vol. 3, New York, Academic Press, 1967, p. 244. O. A. Trowell, "The culture of mature organs in a synthetic medium," Exp. , 16: 118, 1959. H. G. Coon, "Clonal stability and phenotypic expression of chick cartilage cells in vitro," Proc. Natl. Acad. , 55: 66, 1966. 24 R. HAY 15. H. G. Coon, "An established cell line of fibrobroblasts from goose cells, " Carnegie Institution Year Book, 67: 421, 1969.

The second of six parallel cell strains was followed from the 12th to the 27th passage. This culture period lasted 56 days. In this experiment the cultures grew at a much faster rate than in the first experiment. The cultures were subcultured twice a week, while the cultures of the first experiment had several growth periods of seven days. After the 27th passage, growth stopped completely. Just as in the first series of cell strains, these six cell strains showed a surprising similarity in the pattern of their parameter values.

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